Isolation and structure of a new antibiotic, indolizomycin, produced by a strain SK2-52 obtained by interspecies fusion treatment.

As reported in our separate papers,1・2)we obtained the strain SK2-52 by protoplast fUsion treatment between non-antibiotic-producing mutants of Streptomyces griseus and S.tenjimariensis and this produced a new antibiotic. In this communication the isolation characteriza一 tion and structural elucidation of the antibiotic, indolizomycin are reported. The strain SK2-52 was cultured at 27°Cfbr 37hours on a rotatory shaker (180 rpm)in a 500-mlHask containing l 10ml ofamedium[2.0% dextrin,2.0%glycero1,0.3%yeast extract, LO% Bacto-Soytone(Dif°Co),0.2%(NH4)2SO4 and O.2%CaCO3, pH 7.4]. Vegetative inoculum